StackMax Microplate Handling System. 7. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Figure 4. Restart the system. Seller Details. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Unlimited breadth of. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. detection cartridge LUM96: 59. 305 reference level saturation. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. . This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax M5. SpectraMax® i3x. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . ZERO BIAS - scores, article reviews, protocol conditions and moreStructured Review. 1 software Download page. pathways and protein activation and expression in one system. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. Danaher Inc spectramax i3x. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 3. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Replicate values were averaged and imported into Design-Expert 8. Find regional support. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. GM3000 Register or Sign In. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. The spectra analysis and K d were determined using Sigmaplot 14. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. 5 Figures from 202 Publications Thermo Fisher Scientific. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax® Microplate Readers and SoftMax® Pro Software. . HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. com. Engineered for high performance, the auto dual injector cartridge includes fail safe features. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. . SpectraMax i3x microplate reader and SoftMax Pro Software protocol. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. viii SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 340PC control panel are shown in boxed Helvetica type. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. After incubation at 37 °C for 1 h, absorbance was measured at 420 nm (SpectraMax i3x; Molecular Devices). . The calculated LOQ values were 0. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Open the catalog to page 1. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Table 1. Note: Additional readers with similar performance are listed. to View Figures. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Explore a wealth of applications with the future-ready SpectraMax i3x. 1 Microplate Data Acquisition & Analysis Software for Windows. Viewing this answer requires an authorized account. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). Fractional occupancy Corrected BRET ratio NanoBRET. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. . SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. . Supplier: Molecular Devices. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. ü; ü. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Supported Windows Operating System: See release notes. Description 1. The chamber. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. My proficiency in techniques such as •PBMC isolation, counting and. Curves were forced through x,y = 0. The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. For wavelengths in the UV range above 220 nm,. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . SpectraMax Quant dsDNA Assay Kits. 5. The M5 can read volumes down to 2uL with the uMax Low. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Prepare your i3/i3x instrument for shipment. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. . *On the SpectraMax i3x reader only. *On the SpectraMax i3x reader only. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. . The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. . . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax Microplate Readers. Find our technical support information along with contact details. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application. Multimode Readers Comparison Chart. Explore PDF for more details. gen) on a Spectramax i3X luminometer. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Download SoftMax® Pro 7. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. , Fisher Scienti fi c, cat. Find regional support. . SpectraMax i3x Register or Sign In. Figure 1. 0. Example: [Drawer]. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 10. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The Results. . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Microplate Readers SpectraMax. 99. Simplified workflow: Add cellular imaging assays in a small footprint. SpectraMax® Microplate Readers and SoftMax® Pro Software. Supplier: Molecular Devices. SpectraMax iD3 and iD5 Register or Sign In. HTRF-compatible SpectraMax Multi-Mode Readers. 0001 0. )wideand65. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. Supported Windows Operating System: See release notes. HeLa cells were plated at 15,000 cells per well in 100 µL. ImageXpress Confocal HT. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. 303 unable to cal dark current. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. SpectraMax ® iD3 and iD5 Multi-Mode . Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. . The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Bioz Stars score: 98/100, based on 1 PubMed citations. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). ZERO BIAS - scores, article reviews, protocol conditions and more. each new lot of plates, to ensure that the center of each well is read. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Detection cartridge modularity allows for easy addition of new read modes within minutes without. All test measurements and calculations are handledPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. . The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 0. o For example: Ex: 485/Em 525/Cutoff 515 4. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. VAL014, right table) assays on the SpectraMax i3x reader are shown below. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. . ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. ü; Instrument Diagnostics Mode (for SpectraMax® i3x, SpectraMax® iD3, SpectraMax® iD5, SpectraMax® Paradigm)Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. R8362. 1 1 10 100 e e e e e e eSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. . Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 2 nM was calculated using SoftMax Pro Software. 1. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 99 to $1999. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Price from $9. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Find our technical support information along with contact details. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. iD5. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. 1. user upgradeable. . The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. No accessories are needed and SoftMax® Pro. SpectraMax i3x Multi-Mode Detection Platform. Description 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Published Dec 16, 2020 | Updated Apr 13, 2023. 4. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. 303 unable to cal dark current. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 304 signal level saturation. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. The corrected absorbance is within 5% of the. Figure 1. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. AlphaScreen. Read height should be optimized for . Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. style. . 58% for the detection module and 2. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. SpectraMax i3x Register or Sign In. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. Figure 4. 価格、見積もり、お近くの販売店を. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 2. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. However, no piece of equipment is immune to breaking down. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 1 and above:. 306 plate air cal. 304 signal level saturation. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). Eliminate time-dependent substrate addition steps. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 1. spectramax i3x - by Bioz Stars , 2023-11. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. ”Figure 3: Sensitivity of the SpectraMax i3x reader in detecting increasing dilutions of the MycoAlert Assay Control. . SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. . +44 118 944 8000. Align the two slots on the bottle holder with the two pegs on the rail. 100 fmol 1536-well (8 µL) 0. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. 020 fmol 0. to View Figures. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 2 Parallel Line Analysis Conc log [ng/ML] RLU. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. 5 µL anti-cAMP-Cryptate. After 2 hours of treatment cells were harvested by centrifugation and cell pellets were lysed in RIPA buffer (20mM Tris-Cl, 150mM NaCl, 0. The SoftMax® Touch Software is installed in. techsupport. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. StakMax Handling Stacker Base Unit. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 0. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. . Bioz Stars score: 98/100, based on 1 PubMed citations. 99. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 1. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Molecular Devices Software. Description. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Download PDF. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. HTRF IP standard curve. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The solutions were thoroughly mixed and then incubated in the dark at 20°C for 2 h. The SpectraMax i3x reader provides the. Amplitude : 1. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. Settings not required by a reader are indicated by ‘---’. Setting iD5 i3x M5 Paradigm Measurement mode Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Fluorescence Polarization Excitation 485 nm monochromator 485 nm 485 nm 485 nmPage 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. eu@moldev. The dual auto injector module. com Check our website for a current listing of worldwide distributors. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The dual auto injector module. Request a Quote for This Setup. 7 mm for all shake intensities. . Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Table 1. ZERO BIAS - scores, article reviews, protocol conditions and more. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Features. The plate was shaken and incubated in the dark for 10 minutes. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Danaher Inc spectramax i3x. 98 / 100 stars. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. . Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. The chamber. . The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. SpectraMaxi3Multi. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Negative samples contained only MycoAlert Assay Buffer. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Molecular Devices ScanLater Western Blot Detection Platform. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax® i3x. ATP (“flash” luminescence using . . User-upgradeable detection modules allow you to expand the capabilities of your microp. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. com. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Actual shake speed is based on the microplate format. 01 0. 12911151) 0. 9000-0756. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. pH-Xtra Glycolysis Assay - Agilent. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. 58% for the detection module and 2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices are known for both the reliability and the wide uses of their products. The SpectraMax i2X is used for cell migration, cell. Figure 3. were performed on a Biomek i7 containing an integrated orbital plate shaker and a SpectraMax i3x plate reader (Molecular Devices). 3: The cuvette chamber. The CellTiter-Glo assay from Promega uses luciferase.